Please use this identifier to cite or link to this item: http://localhost/handle/Hannan/648702
Title: Calibration of Trapping Force on Cell-Size Objects From Ultrahigh-Frequency Single-Beam Acoustic Tweezer
Authors: Hae Gyun Lim;Ying Li;Ming-Yi Lin;Changhan Yoon;Changyang Lee;Hayong Jung;Robert H. Chow;K. Kirk Shung
subject: ultrahigh-frequency focused ultrasound|Acoustic trapping force|calibration|micropipette aspiration|trap stiffness
Year: 2016
Publisher: IEEE
Abstract: In this paper, we report a calibration of acoustic trapping force of single-beam acoustic tweezer (SBAT) at ultrahigh frequency using micropipette aspiration. The acoustic trapping forces (F<sub>trapping</sub>) and the trap stiffness on a 5-&#x03BC;m polystyrene microbead for a 110-MHz SBAT were measured against the known force generated from a micropipette. The trap stiffness (k), which represents F<sub>trapping</sub> corresponding to a displacement (x) of a microbead from the trap center, was measured and the results showed that a higher duty factor and excitation voltage lead to a stronger trapping force and trap stiffness for a given displacement. Since a precisely calibrated force generated from a micropipette is directly applied to the calculation of acoustic trapping force, the approach should be more flexible than those previously reported. In addition, with this method, precisely controlling the tip size of a micropipette within a few micrometers allows the possibility of calibrating the trapping force on an object of the size of a single cell. It not only helps better evaluate the trapping performance of SBAT as a tool of cell manipulation, but also helps develop SBAT as a useful tool for assessing cellular interactions.
Description: 
URI: http://localhost/handle/Hannan/178370
http://localhost/handle/Hannan/648702
ISSN: 0885-3010
volume: 63
issue: 11
Appears in Collections:2016

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Title: Calibration of Trapping Force on Cell-Size Objects From Ultrahigh-Frequency Single-Beam Acoustic Tweezer
Authors: Hae Gyun Lim;Ying Li;Ming-Yi Lin;Changhan Yoon;Changyang Lee;Hayong Jung;Robert H. Chow;K. Kirk Shung
subject: ultrahigh-frequency focused ultrasound|Acoustic trapping force|calibration|micropipette aspiration|trap stiffness
Year: 2016
Publisher: IEEE
Abstract: In this paper, we report a calibration of acoustic trapping force of single-beam acoustic tweezer (SBAT) at ultrahigh frequency using micropipette aspiration. The acoustic trapping forces (F<sub>trapping</sub>) and the trap stiffness on a 5-&#x03BC;m polystyrene microbead for a 110-MHz SBAT were measured against the known force generated from a micropipette. The trap stiffness (k), which represents F<sub>trapping</sub> corresponding to a displacement (x) of a microbead from the trap center, was measured and the results showed that a higher duty factor and excitation voltage lead to a stronger trapping force and trap stiffness for a given displacement. Since a precisely calibrated force generated from a micropipette is directly applied to the calculation of acoustic trapping force, the approach should be more flexible than those previously reported. In addition, with this method, precisely controlling the tip size of a micropipette within a few micrometers allows the possibility of calibrating the trapping force on an object of the size of a single cell. It not only helps better evaluate the trapping performance of SBAT as a tool of cell manipulation, but also helps develop SBAT as a useful tool for assessing cellular interactions.
Description: 
URI: http://localhost/handle/Hannan/178370
http://localhost/handle/Hannan/648702
ISSN: 0885-3010
volume: 63
issue: 11
Appears in Collections:2016

Files in This Item:
File Description SizeFormat 
7544585.pdf1.59 MBAdobe PDFThumbnail
Preview File
Title: Calibration of Trapping Force on Cell-Size Objects From Ultrahigh-Frequency Single-Beam Acoustic Tweezer
Authors: Hae Gyun Lim;Ying Li;Ming-Yi Lin;Changhan Yoon;Changyang Lee;Hayong Jung;Robert H. Chow;K. Kirk Shung
subject: ultrahigh-frequency focused ultrasound|Acoustic trapping force|calibration|micropipette aspiration|trap stiffness
Year: 2016
Publisher: IEEE
Abstract: In this paper, we report a calibration of acoustic trapping force of single-beam acoustic tweezer (SBAT) at ultrahigh frequency using micropipette aspiration. The acoustic trapping forces (F<sub>trapping</sub>) and the trap stiffness on a 5-&#x03BC;m polystyrene microbead for a 110-MHz SBAT were measured against the known force generated from a micropipette. The trap stiffness (k), which represents F<sub>trapping</sub> corresponding to a displacement (x) of a microbead from the trap center, was measured and the results showed that a higher duty factor and excitation voltage lead to a stronger trapping force and trap stiffness for a given displacement. Since a precisely calibrated force generated from a micropipette is directly applied to the calculation of acoustic trapping force, the approach should be more flexible than those previously reported. In addition, with this method, precisely controlling the tip size of a micropipette within a few micrometers allows the possibility of calibrating the trapping force on an object of the size of a single cell. It not only helps better evaluate the trapping performance of SBAT as a tool of cell manipulation, but also helps develop SBAT as a useful tool for assessing cellular interactions.
Description: 
URI: http://localhost/handle/Hannan/178370
http://localhost/handle/Hannan/648702
ISSN: 0885-3010
volume: 63
issue: 11
Appears in Collections:2016

Files in This Item:
File Description SizeFormat 
7544585.pdf1.59 MBAdobe PDFThumbnail
Preview File